Performance and population analysis of hydrogen production from sugarcane juice by non-sterile continuous stirred tank reactor augmented with Clostridium butyricum
✍ Scribed by Sakchai Pattra; Chyi-How Lay; Chiu-Yue Lin; Sompong O-Thong; Alissara Reungsang
- Book ID
- 103832461
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 433 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0360-3199
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✦ Synopsis
Non-sterile operation of continuous stirred tank reactor (CSTR) augmented with Clostridium butyricum and fed with sugarcane juice was studied at various hydraulic retention time (HRT). The maximum hydrogen production rate and yield of 3.38 mmol H 2 /L/h and 1.0 mol H 2 /mol hexose consumed, respectively, were achieved at HRT 4 h. The relationship of the augmented microorganism and normal flora in the fermentation system under non-sterile condition were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Initially, at HRT 36 h, other species related to Lactobacillus harbinensis and Klebseilla pneumoniae were present as a major group in the reactor. When HRT was decreased to 12, 6 and 4 h, C. butyricum was present with a competition between L. harbinensis and K. pneumoniae. Results indicated that augmented C. butyricum could compete with contaminated microorganisms during non-sterile operation at low HRT (12e4 h) with the support of normal flora (K. pneumoniae).