๐”– Bobbio Scriptorium
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Perforin binding to cells and lipid membranes determined by a simple competition assay

โœ Scribed by Shibo Jiang; David M. Ojcius; J.D.-E. Young

Book ID
118901754
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
680 KB
Volume
126
Category
Article
ISSN
0022-1759

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A simple, rapid assay for measuring radiolabeled ligand binding to purified plasma membranes was developed. In this assay, membrane proteins and ligand are mixed atop a nonmiscible silicone oil (density = 1.029 g/cm9 and incubated to establish equilibrium. The membrane proteins and bound ligand are