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Perchlorate-induced conformational transition of Staphylococcal nuclease: evidence for an equilibrium unfolding intermediate

โœ Scribed by Haripada Maity; Maurice R. Eftink


Book ID
116177108
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
394 KB
Volume
431
Category
Article
ISSN
0003-9861

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Fluorescence and circular dichroism data as a function of temperature were obtained to characterize the unfolding of nuclease A and two of its less stable mutants. These spectroscopic data were obtained with a modified instrument that enables the nearly simultaneous detection of both fluorescence an