Peptide synthesis during in vitro proteolysis - transpeptidation or condensation?

Evidence for peptide synthesis in the co

Evidence for peptide synthesis in the course of in vitro proteolysis

✍ Lorenzen, P. Chr. ;Goepfert, A. ;Schieber, A. ;Brückner, H. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 504 KB

The present paper reports on studies concerned with furnishing evidence for peptide synthesis in the course of in vitro proteolysis. To this end, the oxidized chain B from insulin (INS) (S = 5 % in demineralized water) was subjected to tryptic proteolysis (E/S = 1/50; pH 5; 37 "C; 24 h). HPLC -as we