Peptide Self-Assembly

✍ Scribed by Bradley L. Nilsson, Todd M. Doran

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 453
• Series: Methods in Molecular Biology 1777
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of self-assembled peptide materials, and structural and mechanistic analyses of pathological amyloid systems that provide novel ways to assess function of the various possible aggregates as well to determine how the structure of these materials correlates to function/dysfunction in the biological context. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Peptide Self-Assembly: Methods and Protocols aims to capture modern methods that span the breadth of the exciting and expanding field of peptide self-assembly.

✦ Table of Contents

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
Methods to Characterize the Nanostructure and Molecular Organization of Amphiphilic Peptide Assemblies (V. Castelletto, I. W. Hamley)....Pages 3-21
Solid-State NMR Structural Characterization of Self-Assembled Peptides with Selective 13C and 15N Isotopic Labels (Danting Huang, Benjamin C. Hudson, Yuan Gao, Evan K. Roberts, Anant K. Paravastu)....Pages 23-68
ATR-FTIR Analysis of Amyloid Proteins (Jean-Marie Ruysschaert, Vincent Raussens)....Pages 69-81
Imaging Protein Fibers at the Nanoscale and In Situ (Angelo Bella, Michael Shaw, Emiliana De Santis, Maxim G. Ryadnov)....Pages 83-100
Replica Exchange Molecular Dynamics: A Practical Application Protocol with Solutions to Common Problems and a Peptide Aggregation and Self-Assembly Example (Ruxi Qi, Guanghong Wei, Buyong Ma, Ruth Nussinov)....Pages 101-119
An Aggregate Weight-Normalized Thioflavin-T Measurement Scale for Characterizing Polymorphic Amyloids and Assembly Intermediates (Ronald Wetzel, Saketh Chemuru, Pinaki Misra, Ravi Kodali, Smita Mukherjee, Karunakar Kar)....Pages 121-144
Nanoparticle Tracking for Protein Aggregation Research (Xiaomeng Lu, Regina M. Murphy)....Pages 145-158
Peptide Self-Assembly Measured Using Fluorescence Correlation Spectroscopy (Judith J. Mittag, Joachim O. Rädler, Jennifer J. McManus)....Pages 159-171
Front Matter ....Pages 173-173
A General Method to Prepare Peptide-Based Supramolecular Hydrogels (Dan Yuan, Junfeng Shi, Ning Zhou, Bing Xu)....Pages 175-180
Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks (Nina Dinjaski, Wenwen Huang, David L. Kaplan)....Pages 181-192
Synthesis of Mikto-Arm Star Peptide Conjugates (Jin Mo Koo, Hao Su, Yi-An Lin, Honggang Cui)....Pages 193-207
Synthesis and Evaluation of Self-Assembled Nanostructures of Peptide-π Chromophore Conjugates (Tejaswini S. Kale, John D. Tovar)....Pages 209-220
Programmable Fabrication of Multilayer Collagen Nanosheets of Defined Composition (Tao Jiang, Vincent P. Conticello)....Pages 221-232
Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies (Carolina Mora Solano, Yi Wen, Huifang Han, Joel H. Collier)....Pages 233-248
Microwave-Assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines (C. Brent Chesson, Rojelio Elias Alvarado, Jai S. Rudra)....Pages 249-259
Preparation and Screening of Catalytic Amyloid Assemblies (Zsofia Lengyel, Caroline M. Rufo, Ivan V. Korendovych)....Pages 261-270
Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery (Dawei Xu, Louis DeRidder, Brendan Elmore, He Dong)....Pages 271-281
Biogelx: Cell Culture on Self-Assembling Peptide Gels (Mhairi M. Harper, Michael L. Connolly, Laura Goldie, Eleanore J. Irvine, Joshua E. Shaw, Vineetha Jayawarna et al.)....Pages 283-303
Front Matter ....Pages 305-305
Production and Use of Recombinant Aβ for Aggregation Studies (Tiernan T. O’Malley, Sara Linse, Dominic M. Walsh)....Pages 307-320
Disaggregation of Aβ42 for Structural and Biochemical Studies (Hyewon Chung, Elliot J. Crooks, Martine Ziliox, Steven O. Smith)....Pages 321-330
Preparation of Stable Amyloid-β Oligomers Without Perturbative Methods (Samuel A. Kotler, Ayyalusamy Ramamoorthy)....Pages 331-338
Discriminating Strains of Self-Propagating Protein Aggregates Using a Conformational Stability Assay (Heather H. C. Lau, Angus Lau, Joel C. Watts)....Pages 339-354
Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption (Wei Qiang, Katelynne E. Doherty)....Pages 355-367
Using Molecular Tweezers to Remodel Abnormal Protein Self-Assembly and Inhibit the Toxicity of Amyloidogenic Proteins (Ravinder Malik, Jing Di, Gayatri Nair, Aida Attar, Karen Taylor, Edmond Teng et al.)....Pages 369-386
Incorporation of an Azobenzene β-Turn Peptidomimetic into Amyloid-β to Probe Potential Structural Motifs Leading to β-Sheet Self-Assembly (Todd M. Doran, Bradley L. Nilsson)....Pages 387-406
Solid-State NMR Studies of Amyloid Materials: A Protocol to Define an Atomic Model of Aβ(1–42) in Amyloid Fibrils (Yiling Xiao, Dan McElheny, Minako Hoshi, Yoshitaka Ishii)....Pages 407-428
Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies (Baiping Ren, Rundong Hu, Mingzhen Zhang, Yonglan Liu, Lijian Xu, Binbo Jiang et al.)....Pages 429-447
Back Matter ....Pages 449-452

✦ Subjects

Life Sciences; Protein Science