Peptide, Protein and Enzyme Design

✍ Scribed by Vincent L. Pecoraro (Eds.)

• Publisher: Academic Press
• Year: 2016
• Tongue: English
• Leaves: 656
• Series: Methods in Enzymology 580
• Edition: 1
• Category: Library

✦ Synopsis

De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction.

• Continues the legacy of this premier serial with quality chapters on de novo enzyme design
• Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field
• Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism

✦ Table of Contents

Content:
Series Page Page ii
Copyright Page iv
Contributors Pages xiii-xvi
Preface Pages xvii-xxii V.L. Pecoraro
Chapter One - Chemical Posttranslational Modification with Designed Rhodium(II) Catalysts Pages 1-19 S.C. Martin, M.B. Minus, Z.T. Ball
Chapter Two - Cell-Binding Assays for Determining the Affinity of Protein–Protein Interactions: Technologies and Considerations Pages 21-44 S.A. Hunter, J.R. Cochran
Chapter Three - Protein and Antibody Engineering by Phage Display Pages 45-87 J.C. Frei, J.R. Lai
Chapter Four - Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian Cells Pages 89-107 R. Serfling, I. Coin
Chapter Five - Method for Enzyme Design with Genetically Encoded Unnatural Amino Acids Pages 109-133 C. Hu, J. Wang
Chapter Six - Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three-Stranded Coiled-Coil Structure Containing d-Amino Acids Pages 135-148 L. Ruckthong, J.A. Stuckey, V.L. Pecoraro
Chapter Seven - SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience Pages 149-167 C. Schoene, S.P. Bennett, M. Howarth
Chapter Eight - Engineering and Application of LOV2-Based Photoswitches Pages 169-190 S.P. Zimmerman, B. Kuhlman, H. Yumerefendi
Chapter Nine - Minimalist Design of Allosterically Regulated Protein Catalysts Pages 191-202 O.V. Makhlynets, I.V. Korendovych
Chapter Ten - Combining Design and Selection to Create Novel Protein–Peptide Interactions Pages 203-222 E.B. Speltz, N. Sawyer, L. Regan
Chapter Eleven - Metal-Directed Design of Supramolecular Protein Assemblies Pages 223-250 J.B. Bailey, R.H. Subramanian, L.A. Churchfield, F.A. Tezcan
Chapter Twelve - Designing Fluorinated Proteins Pages 251-278 E.N.G. Marsh
Chapter Thirteen - Solid Phase Synthesis of Helically Folded Aromatic Oligoamides Pages 279-301 S.J. Dawson, X. Hu, S. Claerhout, I. Huc
Chapter Fourteen - Conformational Restriction of Peptides Using Dithiol Bis-Alkylation Pages 303-332 L. Peraro, T.R. Siegert, J.A. Kritzer
Chapter Fifteen - Engineering Short Preorganized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case Study Pages 333-364 L.M.P. Lima, O. Iranzo
Chapter Sixteen - De Novo Construction of Redox Active Proteins Pages 365-388 C.C. Moser, M.M. Sheehan, N.M. Ennist, G. Kodali, C. Bialas, M.T. Englander, B.M. Discher, P.L. Dutton
Chapter Seventeen - Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen Production Pages 389-416 R. Alcala-Torano, D.J. Sommer, Z. Bahrami Dizicheh, G. Ghirlanda
Chapter Eighteen - Equilibrium Studies of Designed Metalloproteins Pages 417-438 B.R. Gibney
Chapter Nineteen - Reconstitution of Heme Enzymes with Artificial Metalloporphyrinoids Pages 439-454 K. Oohora, T. Hayashi
Chapter Twenty - Creation of a Thermally Tolerant Peroxidase Pages 455-470 Y. Watanabe, H. Nakajima
Chapter Twenty-One - Designing Covalently Linked Heterodimeric Four-Helix Bundles Pages 471-499 M. Chino, L. Leone, O. Maglio, A. Lombardi
Chapter Twenty-Two - Design of Heteronuclear Metalloenzymes Pages 501-537 A. Bhagi-Damodaran, P. Hosseinzadeh, E. Mirts, J. Reed, I.D. Petrik, Y. Lu
Chapter Twenty-Three - Periplasmic Screening for Artificial Metalloenzymes Pages 539-556 M. Jeschek, S. Panke, T.R. Ward
Chapter Twenty-Four - De Novo Designed Imaging Agents Based on Lanthanide Peptides Complexes Pages 557-580 A.F.A. Peacock
Chapter Twenty-Five - Peptide Binding for Bio-Based Nanomaterials Pages 581-598 N.M. Bedford, C.J. Munro, M.R. Knecht
Author Index Pages 599-637
Subject Index Pages 639-653

✦ Subjects

Peptides;Enzymes;SCIENCE;Life Sciences;Biochemistry