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Peptide motifs of HLA-B58, B60, B61, and B62 molecules

✍ Scribed by Kirsten Falk; Olaf Rötzschke; Masafumi Takiguchi; Volker Gnau; Stefan Stevanović; Günther Jung; Hans-Georg Rammensee

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
285 KB
Volume
41
Category
Article
ISSN
0093-7711

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✦ Synopsis

gene used for transfection had been cloned from a Japanese individual and was confirmed by sequencing to be B'I501 (M. Takiguchi and co-workers, unpublished results). Transfectants were expanded in about 30 to 50 1 of roller bottle cultures. Cell pellets of 20 to 50 ml were detergent lysed, and the HLA molecules precipitated, using HLA-A-, B-, Cspecific W6/32 antibodies (Barnstable et al. 1978), as described previously (Falk et al. 1991). Peptides were dissociated from HLA molecules by treatment with 0.1% TFA and separated on a 2.1 xl00 mm reversed phase C2/ C18 HPLC column (Pharmacia, Freiburg, Germany); using the SMART system (Pharmacia). Material eluting in dis-

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