Analysis of lipid hydrolytic activity by
β
Youji Shimazaki; Masayuki Miyamoto
π
Article
π
2007
π
John Wiley and Sons
π
English
β 190 KB
## Abstract After separation by microscale nonβdenaturing twoβdimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t