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Peptide mapping of polypeptides separated by two-dimensional electrophoresis: Protease digestion directly on the two-dimensional gel followed by electrophoresis in reverse direction

✍ Scribed by Michel Zivy; Fabienne Granier


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
591 KB
Volume
9
Category
Article
ISSN
0173-0835

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## Abstract After separation by microscale non‐denaturing two‐dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t