Pentose phosphate pathway mutants of yeast
β Scribed by Lobo, Zita ;Maitra, P. K.
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 198 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0026-8925
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π SIMILAR VOLUMES
Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type s
The in vivo dynamics of the pentose phosphate pathway has been studied with transient experiments in continuous culture of Saccaromyces cerevisiae. Rapid sampling was performed with a special sampling device after disturbing the steady state with a pulse of glucose. The time span of observation was