Penetration of zona-free hamster eggs in vitro by goat spermatozoa preincubated in the reproductive tract isolated from a maturing gilt
✍ Scribed by Kim, C. I. ;Niwa, K. ;Imai, H. ;Iritani, A.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 273 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
When goat spermatozoa were preincubated for 5‐5.5 hr in the isolated uterus and oviduct from a maturing gilt, they developed the ability to penetrate zona‐free hamster eggs in vitro. Overall penetration rates were 17% and 0%, respectively, 1 hr after insemination, and these increased to 59% and 54% 2 hr later. The penetration rates were not improved by incubation longer than 2 hr after insemination up to 5 hr. Goat spermatozoa preincubated for 5 hr in a m‐KRB solution with or without goat serum penetrated zona‐free hamster eggs with difficulty. The results suggest that a functional change in the ability of goat spermatozoa to penetrate zona‐free hamster eggs may occur in the reproductive tract of different species.