Penetration of glucose into the human red cell: The recent attack on the carrier hypothesis
✍ Scribed by LeFevre, Paul G.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1959
- Tongue
- English
- Weight
- 262 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0095-9898
No coin nor oath required. For personal study only.
✦ Synopsis
T W O FIGURES
A sizable body of evidence accumulated during the last decade indicates that simple sugars pass into the human erythrocyte not by simply diffusing through the cell membrane, but by forming a transitory combination with some special surface component (reviewed by LeFevre, '54, and Wilbrandt, '54 ; confirmed by Widdas, '54 ; Bowyer and Widdas, '56, and Reinwein et al., '57). Mawe ( '56) rejects the kinetic aspects of this evidence, citing new densimetric and chemical experiments as proof that the entry of glucose into properly handled red cells is adequately described by the laws of diffusion. The present note challenges this conclusion on the grounds that (1) recalculation from Mawe's hematocrit and chemical analytic data supports the carrier interpretation; and (2) new experiments incorporating Mawe's procedural recommendations still contradict his densimetric results.
The chemical ezperiments
Mawe rapidly separated the cells and plasma a t intervals after adding glucose-enriched plasma to concentrated whole blood, and determined the hematocrit and plasma glucose levels. The one complete experiment described (fig. 7 of Mawe's paper) is kinetically far more consistent with the *Research supported by the U. 8. Atomic Energy Commission.