Pendred syndrome and DFNB4-mutation screening of SLC26A4 by denaturing high-performance liquid chromatography and the identification of eleven novel mutations

Rapid identification and screening of pr

Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by multi-assisted laser desorption/ionization mass spectrometry analysis and sequence database searching

✍ Yajuan Chen; Dan Wall; David M. Lubman 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 97 KB 👁 2 views

Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100