Peculiar allelotype associated with susceptibility to neuroblastoma

✍ Scribed by Patrizia Perri; Annalisa Pession; Katia Mazzocco; Paolo Strigini; Achille Iolascon; Giuseppe Basso; Gian Paolo Tonini

• Book ID: 101259957
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 378 KB
• Volume: 17
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199609)17:1<60::aid-gcc9>3.0.co;2-0

✦ Synopsis

Human neuroblastoma (NB) is characterized genetically by deletions of the short arm of chromosome I and by MYCN amplification. Loss of heterozygosity (LOH) has been found frequently for region I p36. We have studied restriction fragment length polymorphisms (RFLPs) by using anonymous and hypervariable region (HVR) sequences to demonstrate LOH for I p loci in 50 Italian neuroblastoma patients. Twelve cases (25%) showed LOH at one or more loci. Locus D1S94 was the most frequently involved (8/ I 2 cases with deletion; 67%). MYCN amplification was observed in 20% of the patients. We also studied the allelic distribution in the constitutional DNA of neuroblastoma patients and of healthy Italian subjects for loci DlSl12 and DIS94. A significantly (P = 0.01) different allele frequency was detected in the two groups at locus DIS94, but not at DISI 12. Furthermore, the NB population was not in Hardy-Weinberg equilibrium at the former locus. This new observation suggests the existence of an allelotype associated with the susceptibility to neuroblastoma.