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Peak shape improvement of basic analytes in capillary liquid chromatography

✍ Scribed by Anja Prüß; Christine Kempter; Jens Gysler; Jürgen Maier-Rosenkranz; Thomas Jira

Book ID
102442211
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
284 KB
Volume
28
Category
Article
ISSN
1615-9306

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✦ Synopsis

Peak shape improvement of basic analytes in capillary liquid chromatography

The analysis of bases is of special interest in pharmaceutical research because numerous active substances contain basic functional groups. Capillary and conventional size LC separations of drug substances spiked with potential impurities were compared. In the case of the nonpolar drug levonorgestrel equal separation efficiency was readily attained by both techniques. The peaks of basic substances, however, showed extensive tailing when separated by capillary LC. The peak deformation was attributable to interactions of the basic substances with the polar inner surface of the fused silica capillaries employed in capillary LC and does not appear with the steel tubing generally used in conventional size LC. This drawback of capillary LC was overcome by use of deactivated fused silica capillaries for column hardware and transfer lines.

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