pDNA/poly(L-lysine) Polyplexes Functionalized with a pH-Sensitive Charge-Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells
✍ Scribed by Mai Sanjoh; Shigehiro Hiki; Yan Lee; Makoto Oba; Kanjiro Miyata; Takehiko Ishii; Kazunori Kataoka
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 395 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1022-1336
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✦ Synopsis
Abstract
An efficient endosome‐escaping function was integrated into the polyplex of plasmid DNA (pDNA) with poly(L‐lysine) (PLys) to improve its gene transfection efficiency through electrostatic coating with charge‐conversional polymer (CCP). CCP showed charge‐conversional function responding to endosomal pH, leading to the release of pDNA/PLys polyplex into the cytoplasm. The cells took up the intact CCP‐integrated ternary polyplex, which exerted appreciably higher transfection efficiency with lower cytotoxicity than pDNA/PLys polyplex against human umbilical vein endothelial cells (HUVECs). This is consistent with the facilitated endosomal escape of the CCP‐integrated ternary polyplex compared to the pDNA/PLys polyplex as directly observed with confocal laser‐scanning microscopy.
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