PCR Sequencing Protocols Volume 65 || DNA Rescue by the Vectorette Method

✍ Scribed by Rapley, Ralph

• Book ID: 120339709
• Publisher: Humana Press
• Year: 1996
• Tongue: English
• Weight: 907 KB
• Category: Article
• ISBN-13: 9780896033443
• DOI: 10.1385/0-89603-344-9:201

✦ Synopsis

Preparation And Analysis Of Dna Sequencing Gels -- Purification Of Pcr Products From Agarose Gels For Direct Sequencing -- Enzymatic Fluorescence And Biotin Labeling Of Primers For Pcr Sequencing -- Direct Sequencing Of Double-stranded Pcr Products With The Sequenase Kit And [alpha-³⁵s] Datp -- Direct Sequencing By Thermal Asymmetric Pcr -- Rapid Sequencing Of Cdna Clones : Direct Sequencing Using Sequential Linear/asymmetric Pcr -- Direct Sequencing Of Pcr Products Using Chemiluninescent Detection -- Direct Dna Sequencing Of Pcr Products Using Magnetic Beads -- Affinity Capture And Solid-phase Sequencing Of Biotinylated Pcr Products -- Analysis Of Nucleotide Sequence Variations By Solid-phase Minisequencing -- Nonradioactive Pcr Sequencing Using Digoxigenin -- Silver Sequencing Nonradioactive Cycle Sequencing Of Dsdna -- Direct Sequencing Of Pcr Products With Dna-binding Proteins -- Pcr Sequencing With The Aid Of Detergents -- Direct Sequencing With Highly Degenerate And Inosine-containing Primers -- Determination Of Unknown Genomic Sequences Without Cloning -- Dna Sequencing By The Chemical Method -- Direct Pcr Sequencing With Denaturants (formamide) -- Efficient Pcr Production Of Single-stranded Dna Sequencing Templates -- Preparation And Direct Automated Cycle Sequencing Of Pcr Products -- Solid-phase Automated Sequencing Of Pcr-ampilified Genomic Dna -- Cloning Pcr Products For Sequencing In M13 Vectors -- Sequencing Pcr Products Cloned Into M13 Vectors -- Genomic Ampilificatin With Transcript Sequencing (gawts) -- Dna Rescue By The Vectorette Method -- Sequencing Of (da:dt) Cloned Mixed Pcr Products From Microbial Populations. Edited By Ralph Rapley. Includes Bibliographical References And Index.