PCR Protocols: Current Methods and Appli
β Bruce A. White
π Library
π
1993
π Humana Press
π English
β¦ LIBER β¦
π
PCR Protocols. A Guide to Methods and Applications
β Scribed by Michael A. Innis, David H. Gelfand, John J. Sninsky and Thomas J. White (Eds.)
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Leaves
- 481
- Category
- Library
β¬ Acquire This Volume
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β¦ Synopsis
The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and Read more...
β¦ Table of Contents
Content:
Front Matter, Page iii
Copyright, Page iv
CONTRIBUTORS, Pages xi-xvi
PREFACE, Pages xvii-xviii, Thomas J. White
1 - OPTIMIZATION OF PCRs, Pages 3-12, Michael A. Innis, David H. Gelfand
2 - AMPLIFICATION OF GENOMIC DNA, Pages 13-20, Randall K. Saiki
3 - AMPLIFICATION OF RNA, Pages 21-27, Ernest S. Kawasaki
4 - RACE: RAPID AMPLIFICATION OF cDNA ENDS, Pages 28-38, Michael A. Frohman
5 - DEGENERATE PRIMERS FOR DNA AMPLIFICATION, Pages 39-45, Teresa Compton
6 - cDNA CLONING USING DEGENERATE PRIMERS, Pages 46-53, Cheng Chi Lee, C. Thomas Caskey
7 - PCR WITH 7-DEAZA-2β²-DEOXYGUANOSINE TRIPHOSPHATE, Pages 54-59, Michael A. Innis
8 - COMPETITIVE PCR FOR QUANTITATION OF mRNA, Pages 60-69, Gary Gilliland, Steven Perrin, H. Franklin Bunn
9 - QUANTITATIVE PCR, Pages 70-75, Alice M. Wang, David F. Mark
10 - PRODUCTION OF SINGLE-STRANDED DNA BY ASYMMETRIC PCR, Pages 76-83, Peter C. McCabe
11 - CLONING WITH PCR, Pages 84-91, Stephen J. Scharf
12 - OLIGONUCLEOTIDE LIGATION ASSAY, Pages 92-98, Ulf Landegren, Robert Kaiser, Leroy Hood
13 - NONISOTOPICALLY LABELED PROBES AND PRIMERS, Pages 99-112, Corey Levenson, Chu-an Chang
14 - INCORPORATION OF BIOTINYLATED dUTP, Pages 113-118, Y-M. Dennis Lo, Wajahat Z. Mehal, Kenneth A. Fleming
15 - NONISOTOPIC DETECTION OF PCR PRODUCTS, Pages 119-128, Rhea Helmuth
16 - THERMOSTABLE DNA POLYMERASES, Pages 129-141, David H. Gelfand, Thomas J. White
17 - PROCEDURES TO MINIMIZE PCR-PRODUCT CARRY-OVER, Pages 142-145, Shirley Kwok
18 - SAMPLE PREPARATION FROM BLOOD, CELLS, AND OTHER FLUIDS, Pages 146-152, Ernest S. Kawasaki
19 - SAMPLE PREPARATION FROM PARAFFIN-EMBEDDED TISSUES, Pages 153-158, Deann K. Wright, M. Michele Manos
20 - AMPLIFYING ANCIENT DNA, Pages 159-166, Svante PÀÀbo
21 - IN VITRO TRANSCRIPTION OF PCR TEMPLATES, Pages 169-176, Michael J. Holland, Michael A. Innis
22 - RECOMBINANT PCR, Pages 177-183, Russell Higuchi
23 - DNase I FOOTPRINTING, Pages 184-188, Barbara Krummel
24 - SEQUENCING WITH Taq DNA POLYMERASE, Pages 189-196, Mary Ann D. Brow
25 - DIRECT SEQUENCING WITH THE AID OF PHAGE PROMOTERS, Pages 197-205, Steve S. Sommer, Gobinda Sarkar, Dwight D. Koeberl, Cynthia D.K. Bottema, Jean-Marie Buerstedde, David B. Schowalter, Joslyn D. Cassady
26 - IDENTIFYING DNA POLYMORPHISMS BY DENATURING GRADIENT GEL ELECTROPHORESIS, Pages 206-218, Val C. Sheffield, David R. Cox, Richard M. Myers
27 - AMPLIFICATION OF FLANKING SEQUENCES BY INVERSE PCR, Pages 219-227, Howard Ochman, Meetha M. Medhora, Dan Garza, Daniel L. Hartl
28 - DETECTION OF HOMOLOGOUS RECOMBINANTS, Pages 228-236, Michael A. Frohman, Gail R. Martin
29 - RNA PROCESSING: Apo-B, Pages 237-244, Lyn M. Powell
30 - A TRANSCRIPTION-BASED AMPLIFICATION SYSTEM, Pages 245-252, T.R. Gingeras, G.R. Davis, K.M. Whitfield, H.L. Chappelle, L.J. DiMichele, D.Y. Kwoh
31 - SCREENING OF Ξ»gt11 LIBRARIES, Pages 253-258, Kenneth D. Friedman, Nancy L. Rosen, Peter J. Newman, Robert R. Montgomery
32 - HLA DNA TYPING, Pages 261-271, Henry A. Erlich, Teodorica L. Bugawan
33 - MULTIPLEX PCR FOR THE DIAGNOSIS OF DUCHENNE MUSCULAR DYSTROPHY, Pages 272-281, Jeffrey S. Chamberlain, Richard A. Gibbs, Joel E. Ranier, C. Thomas Caskey
34 - ISOLATION OF DNA FROM FUNGAL MYCELIA AND SINGLE SPORES, Pages 282-287, Steven B. Lee, John W. Taylor
35 - GENETIC PREDICTION OF HEMOPHILIA A, Pages 288-299, Scott C. Kogan, Jane Gitschier
36 - HAPLOTYPE ANALYSIS FROM SINGLE SPERM OR DIPLOID CELLS, Pages 300-306, Ulf Gyllensten
37 - AMPLIFICATION OF RIBOSOMAL RNA GENES FOR MOLECULAR EVOLUTION STUDIES, Pages 307-314, Mitchell L. Sogin
38 - AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS, Pages 315-322, T.J. White, T. Bruns, S. Lee, J. Taylor
39 - DETECTION OF HUMAN T-CELL LYMPHOMA/LEUKEMIA VIRUSES, Pages 325-336, Garth D. Ehrlich, Steven Greenberg, Mark A. Abbott
40 - DETECTION OF HUMAN IMMUNODEFICIENCY VIRUS, Pages 337-347, David E. Kellog, Shirley Kwok
41 - DETECTION OF HEPATITIS B VIRUS, Pages 348-355, I. Baginski, A. Ferrie, R. Watson, D. Mack
42 - DETECTION AND TYPING OF GENITAL HUMAN PAPILLOMAVIRUSES, Pages 356-367, Yi Ting, M. Michele Manos
43 - DETECTION OF HUMAN CYTOMEGALOVIRUS, Pages 368-371, Darryl Shibata
44 - PCR AMPLIFICATION OF ENTEROVIRUSES, Pages 372-377, Harley A. Rotbart
45 - NOVEL VIRUSES, Pages 378-385, David Mack, Oh-Sik Kwon, Fred Faloona
46 - ANALYSIS OF ras GENE POINT MUTATIONS BY PCR AND OLIGONUCLEOTIDE HYBRIDIZATION, Pages 386-391, John Lyons
47 - B-CELL LYMPHOMA: t(14;18) CHROMOSOME REARRANGEMENT, Pages 392-398, Marco Crescenzi
48 - DETECTING BACTERIAL PATHOGENS IN ENVIRONMENTAL WATER SAMPLES BY USING PCR AND GENE PROBES, Pages 399-406, Ronald M. Atlas, Asim K. Bej
49 - PCR IN THE DIAGNOSIS OF RETINOBLASTOMA, Pages 407-415, Sang-Ho Park
50 - DETERMINATION OF FAMILIAL RELATIONSHIPS, Pages 416-426, CristiΓ‘n Orrego, Mary Claire King
51 - PCR IN A TEACUP: A Simple and Inexpensive Method for Thermocycling PCRs, Pages 429-434, Robert Watson
52 - A LOW-COST AIR-DRIVEN CYCLING OVEN, Pages 435-441, Peter Denton, H. Reisner
53 - MODIFICATION OF A HISTOKINETTE FOR USE AS AN AUTOMATED PCR MACHINE, Pages 442-446, N.C.P. Cross, N.S. Foulkes, D. Chappel, J. McDonnell, L. Luzzatto
54 - ORGANIZING A LABORATORY FOR PCR WORK, Pages 447-454, CristiΓ‘n Orrego
55 - BASIC EQUIPMENT AND SUPPLIES, Pages 455-459, Roberta Madej, Stephen Scharf
INDEX, Pages 461-482
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