PCR Protocols
β John M. S. Bartlett, David Stirling (auth.), John M. S. Bartlett, David Stirling
π Library
π
2003
π Humana Press
π English
β¦ LIBER β¦
π
PCR Protocols
β Scribed by Zaheer Khan (auth.), Daniel J. Park (eds.)
- Publisher
- Humana Press
- Year
- 2011
- Tongue
- English
- Leaves
- 350
- Series
- Methods in Molecular Biology 687
- Edition
- 3
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, βDNA fingerprintingβ, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biologyβ’ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.
β¦ Table of Contents
Front Matter....Pages i-xi
Front Matter....Pages 1-1
Front Matter....Pages 3-14
Front Matter....Pages 15-15
Front Matter....Pages 17-23
Front Matter....Pages 25-42
Front Matter....Pages 43-55
Back Matter....Pages 57-73
....Pages 75-88
β¦ Subjects
Human Genetics; Nucleic Acid Chemistry; Laboratory Medicine
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β John M. S. Bartlett, David Stirling (auth.), John M. S. Bartlett, David Stirling
π Library
π
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π Humana Press
π English
Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expand