✦ LIBER ✦

PCR Mutation Detection Protocols

✍ Scribed by Andrea M. Guilliatt (auth.), Bimal D. M. Theophilus, Ralph Rapley (eds.)

Book ID: 127438699
Publisher: Humana
Year: 2002
Tongue: English
Weight: 1 MB
Edition: 1
Category: Library
City: Totowa, N.J
ISBN-13: 9781592592739
DOI: 10.1385/1592592732

No coin nor oath required. For personal study only.

✦ Synopsis

Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

✦ Subjects

Cell Biology

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