PCR-based diagnosis of the Filipino (??FIL) and Thai (??THAI) ?-thalassemia-1 deletions

✍ Scribed by Eng, Barry; Patterson, Margaret; Borys, Susan; Chui, David H.K.; Waye, John S.

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 49 KB
• Volume: 63
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/(sici)1096-8652(200001)63:1<54::aid-ajh12>3.0.co;2-b

✦ Synopsis

In southeast Asia, the carrier frequency of two-gene ␣-thalassemia deletions is quite high, ranging from 4% to 14% depending on the population. The most common ␣-thalassemia-1 deletion is the so-called southeast Asian deletion (--SEA ). In addition, a significant proportion of cases involve two other deletions, the Filipino (--FIL ) and Thai (--THAI ) deletions. In this report, we identify the deletion breakpoints for the (--FIL ) and (--THAI ) deletions, and describe PCR-based protocols for rapid and reliable DNA diagnosis of these deletions. Am.