𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Pc 4 photodynamic therapy of U87-derived human glioma in the nude rat

✍ Scribed by John E. George III; Yusra Ahmad; Davood Varghai; Xiaolin Li; Jeffrey Berlin; David Jackowe; Marc Jungermann; Michael S. Wolfe; Lothar Lilge; Ali Totonchi; Rachel L. Morris; Allyn Peterson; W. David Lust; Malcolm E. Kenney; Charles L. Hoppel; Jiayang Sun; Nancy L. Oleinick; David Dean

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
200 KB
Volume
36
Category
Article
ISSN
0196-8092

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background and Objectives

As a potential therapy for malignant glioma, we tested the phthalocyanine photosensitizer Pc 4 for: (1) rapid clearance from the vasculature, (2) specificity for glioma, and (3) tumoricidal photosensitizing capability.

Study Design/Materials and Methods

Parenchymal injection of U87 cells into athymic rat brains (N = 100) was followed after 12 days by tail vein injection of 0.5 mg/kg Pc 4. After 1 day, the tumor was illuminated with either 5 (N = 11) or 30 (N = 16) J/cm^2^ red light at 672 nm. Sacrifice was 1 day later. The brains from these 27 animals underwent H&E (necrosis) and TUNEL assay (apoptosis) histology. Pc 4 concentration of explanted brains and tumors (N = 16), and all blood samples (N = 52) were determined by HPLC‐MS 1 day post Pc 4 administration.

Results

Tumor‐specific apoptosis was almost uniformly seen; however, necrosis was found mostly in the high‐light‐dose group. Pc 4 concentration in bulk tumor averaged 3.8 times greater than in normal brain.

Conclusions

These results warrant expanding this pre‐clinical study to seek effective baseline Pc 4 drug‐ and light‐doses and infusion‐to‐photoirradiation timing that would be necessary for a Pc 4‐mediated PDT clinical trial for glioma patients. Lasers Surg. Med. 36:383–389, 2005. © 2005 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Photodynamic therapy of U87 human glioma
Photodynamic therapy of U87 human glioma in nude rat using liposome-delivered photofrin
✍ Jiang, Feng; Lilge, Lothar; Grenier, Joseph; Li, Yi; Wilson, Mark D.; Chopp, Mic 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 276 KB

## Background and objective: Liposomes as photosensitizer carriers may enhance the photodynamic effect on tumors. ## Study design/materials and methods: To test this hypothesis, we treated u87 human glioma in rat brain with photodynamic therapy (pdt) using photofrin encapsulated in a liposome car

High-field magnetic resonance imaging of
High-field magnetic resonance imaging of the response of human prostate cancer to Pc 4-based photodynamic therapy in an animal model
✍ Baowei Fei; Hesheng Wang; Joseph D. Meyers; Denise K. Feyes; Nancy L. Oleinick; 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 265 KB

## Abstract ## Introduction High‐field magnetic resonance imaging (MRI) is an emerging technique that provides a powerful, non‐invasive tool for __in vivo__ studies of cancer therapy in animal models. Photodynamic therapy (PDT) is a relatively new treatment modality for prostate cancer, the second

Sensitizer for photodynamic therapy of c
Sensitizer for photodynamic therapy of cancer: A comparison of the tissue distribution of photofrin ii and aluminum phthalocyanine tetrasulfonate in nude mice bearing a human malignant tumor
✍ Q. Peng; J. Moan; M. Kongshaug; Jan F. Evensen; H. Anholt; C. Rimington 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 711 KB

## Abstract The distribution of Photofrin II (P‐II) and aluminum phthalocyanine tetrasulfonate (AIPCS~4~) in tissues of BALB/c nu/nu nude mice bearing the LOX human melanoma was measured fluorimetrically at different times after intraperitoneal injection of the drugs, 20 mg/kg body weight. The plas