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pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12

✍ Scribed by Claire M. Berg; Lin Liu; Michael Coon; Linda D. Strausbaugh; Paul Gray; Narendra B. Vartak; Mary Brown; Duncan Talbot; Douglas E. Berg

Book ID: 119079004
Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 300 KB
Volume: 21
Category: Article
ISSN: 0147-619X
DOI: 10.1016/0147-619x(89)90057-7

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Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives

✍ Lopilato, Jane ;Bortner, Scott ;Beckwith, Jon 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 711 KB

We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 rain on the E. coli chromosome. This locus is distinct from