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Patterns of peptide synthesis in senescent and presenescent human fibroblasts

✍ Scribed by Dean L. Engelhardt; Gloria T-Y. Lee; Jeffrey F. Moley

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 457 KB
Volume: 98
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040980121

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Peptide production in senescent and presenescent human foreskin fibroblasts was measured using 2‐dimensional polyacrylamide gel electrophoresis. This procedure permits the visualization of a cohort of the major peptides being produced. Among this cohort of over 500 peptides only two were found to differ in relative amount in that more was being produced in senescent cells. This difference was confirmed by measurements of the relative intensity of the peptide spot. This difference was senescent cell‐specific and not due to the differences in rate of growth of senescent and non‐senescent cells.

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