Patterned glass surfaces direct cell adhesion and process outgrowth of primary neurons of the central nervous system

✍ Scribed by Saneinejad, Samar ;Shoichet, Molly S.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 440 KB
• Volume: 42
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199810)42:1<13::aid-jbm3>3.0.co;2-r

✦ Synopsis

Glass surfaces were patterned with cell-adhesive regions of laminin adhesive peptides YIGSR, RGD, and IK-VAV, and cell-repulsive regions of poly(ethylene glycol) (PEG). The patterns were created by sputter-coating titanium and then gold onto glass coverslips through electron microscope grids. Gold surfaces were modified with cysteine-terminated peptides to have approximately 450 fmol/ cm 2 of peptide incorporated on the glass coverslips as determined with radiolabeled CGYIGSR. Amine-functionalized glass coverslips were prepared using an aminefunctionalized silane and then further modified with PEGaldehyde by a Schiff base reduction. All surfaces were characterized by X-ray photoelectron spectroscopy and water contact angles. Hippocampal neurons, plated from a serumfree medium, adhered preferentially to peptide-functionalized surfaces over PEG-modified surfaces. Cell adhesion and neurite outgrowth were limited to the peptide region, demonstrating that neurite outgrowth could be directed by a combination of cell-adhesive and cell-repulsive cues.