Pathway and products of CO2-fixation by green prokaryotic algae in the cloacal cavity ofDiplosoma virens
✍ Scribed by T. Akazawa; E. H. Newcomb; C. B. Osmond
- Publisher
- Springer-Verlag
- Year
- 1978
- Tongue
- English
- Weight
- 748 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0025-3162
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✦ Synopsis
Light-dependent 14CO2 fixation by the algae of Diplosoma virens (Hartmeyer) ranged between about 3 and 27 ~moles mg-1 chlorophyll h-1. The principal first products of 14C fixation were 3-phosphoglyceric acid and phosphorylated sugars, indicating that ribulose bisphosphate carboxylase was the primary carboxylation enzyme. The activity of this enzyme in crude extracts of the algae was 4 to 6 ~moles CO 2 mg-1 chlorophyll h -I. The principal end product of 14C fixation by these algae in the ascidian host was a water-soluble oligosaccharide which was an ~-1,4-glucan. A maximum of 7% of the 14C fixed was found in insoluble materials of the algae or its host after 60 min 14CO 2 fixation. Whether the ~-1,4-glucan is a product of algal or animal metabolism remains to be determined.