Pathway Analysis and Optimization in Metabolic Engineering

✍ Scribed by Néstor V. Torres, Eberhard O. Voit

• Publisher: Cambridge University Press
• Year: 2002
• Tongue: English
• Leaves: 324
• Edition: 1
• Category: Library

✦ Synopsis

This book introduces researchers and advanced students in biology and engineering to methods of optimizing biochemical systems of biotechnological relevance. It examines the development of strategies for manipulating metabolic pathways, demonstrates the need for effective systems models, and discusses their design and analysis, while placing special emphasis on optimization. The authors propose power-law models and methods of Biochemical Systems Theory toward these ends. All concepts are derived from first principles, and the text is richly illustrated with numerous graphs and examples throughout.

✦ Table of Contents

Contents......Page 8
Preface......Page 10
CRITERIA FOR MODEL SELECTION......Page 16
MODELS OF BIOCHEMICAL PROCESSES......Page 21
SUMMARY......Page 50
2 Methods of Biochemical Systems Theory......Page 57
MODEL DESIGN......Page 58
DESIGN OF EQUATIONS IN BST......Page 61
PARAMETER ESTIMATION......Page 63
DYNAMIC ANALYSIS......Page 71
STEADY-STATE ANALYSIS......Page 75
STABILITY ANALYSIS......Page 79
EVALUATION AND INTERPRETATION......Page 82
INTRODUCTION......Page 90
BASIC CHEMISTRY AND BIOCHEMISTRY OF CITRIC ACID......Page 91
A FIRST MATHEMATICAL MODEL......Page 94
REVISED MODEL......Page 120
QUALITY ASSESSMENT OF THE REVISED MODEL......Page 141
4 Optimization Methods......Page 149
ANALYTICAL METHODS......Page 150
SEARCH ALGORITHMS......Page 168
INTRODUCTION......Page 177
ARE NONLINEARITIES REALLY NECESSARY?......Page 181
SEARCHING FOR EXTREMA OF LINEAR FUNCTIONS WITH MANY VARIABLES......Page 184
OPTIMIZATION OF STOICHIOMETRIC NETWORKS......Page 190
OPTIMIZATION OF S-SYSTEMS......Page 192
OPTIMIZATION OF GMA SYSTEMS......Page 195
MULTIPLE CRITERIA OPTIMIZATION......Page 197
OPTIMIZATION OF STRUCTURE......Page 204
INTRODUCTION......Page 212
A LINEAR PROGRAM FOR OPTIMIZED CITRIC ACID PRODUCTION IN......Page 214
OPTIMAL SOLUTIONS......Page 222
SYSTEMATIC SEARCH FOR THE BEST SEQUENCE OF STEPS TOWARD THE OPTIMAL SOLUTION......Page 225
ANALYSIS OF THE EFFECTS OF IMPRECISION DURING IMPLEMENTATION......Page 229
CONCLUSIONS......Page 237
INTRODUCTION......Page 242
BACKGROUND......Page 244
KINETIC MODEL......Page 246
S-SYSTEM MODEL......Page 249
QUALITY ASSESSMENT OF THE MODEL......Page 250
OPTIMIZATION: THE INDIRECT OPTIMIZATION METHOD (IOM)......Page 253
RESULTS......Page 259
QUALITY ASSESSMENT OF THE INDIRECT OPTIMIZATION METHOD......Page 267
MULTI-OBJECTIVE OPTIMIZATION......Page 273
DISCUSSION......Page 282
8 Conclusions......Page 288
L-CARNITINE PRODUCTION IN HIGH-DENSITY CULTURES OF......Page 289
TRYPTOPHAN BIOSYNTHESIS IN......Page 292
TRENDS IN PATHWAY ANALYSIS AND THE ROLE OF GENE TECHNOLOGY AND GENOMICS......Page 294
THE FUTURE HAS BEGUN......Page 301
Author Index......Page 306
Subject Index......Page 314