Pathogenic Yersinia: Methods and Protocols

✍ Scribed by Viveka Vadyvaloo, Matthew B. Lawrenz

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 259
• Series: Methods in Molecular Biology 2010
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
An Intradermal Model for Yersinia pestis Inoculation (Rodrigo J. Gonzalez)....Pages 3-15
Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis (Roger D. Pechous)....Pages 17-28
Standardized Method for Aerosol Challenge of Rodents with Yersinia pestis for Modeling Primary Pneumonic Plague (Paul E. Anderson, Rachel M. Olson, Joshua L. Willix, Deborah M. Anderson)....Pages 29-39
Mouse Models of Yersiniosis (Diana Hooker-Romero, Leah Schwiesow, Yahan Wei, Victoria Auerbuch)....Pages 41-53
Front Matter ....Pages 55-55
Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-Transmitted Yersinia pestis (Jeffrey G. Shannon, B. Joseph Hinnebusch)....Pages 57-68
Laser Scanning Microscopy of Yersinia pestis Infected Tissues (Rodrigo J. Gonzalez)....Pages 69-84
Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging (Anne Derbise, Olivier Dussurget, Elisabeth Carniel, Javier Pizarro-Cerdá)....Pages 85-97
Discovering Yersinia–Host Interactions by Tissue Dual RNA-Seq (Maria Kusmierek, Ann Kathrin Heroven, Michael Beckstette, Aaron M. Nuss, Petra Dersch)....Pages 99-116
Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using β-Lactamase Fusions (Giang T. Nguyen, Anne L. McCabe, Alyssa C. Fasciano, Joan Mecsas)....Pages 117-139
Monitoring of Neutrophil Recruitment to Mice Lungs During Pneumonic Plague (Yaron Vagima, Yinon Levy, Emanuelle Mamroud)....Pages 141-150
Front Matter ....Pages 151-151
Co-infection Assay to Determine Yersinia pestis Competitive Fitness in Fleas (Athena Lemon, Amelia Silva-Rohwer, Janelle Sagawa, Viveka Vadyvaloo)....Pages 153-166
Drosophila as a Model for Understanding the Insect Host of Yersinia pestis (Anna B. Ludlow, Cassandra D. Pauling, Melanie M. Marketon, Deborah M. Anderson)....Pages 167-178
Front Matter ....Pages 179-179
Intracellular Assays to Monitor Survival and Growth of Yersinia pestis Within Macrophages (Amanda R. Pulsifer, Tiva T. VanCleave, Matthew B. Lawrenz)....Pages 181-196
Human Neutrophil Isolation and Degranulation Responses to Yersinia pestis Infection (Kara R. Eichelberger, William E. Goldman)....Pages 197-209
Measurement of Yersinia Translocon Pore Formation in Erythrocytes (Tiago R. D. Costa, Monika K. Francis, Salah I. Farag, Tomas Edgren, Matthew S. Francis)....Pages 211-229
Analysis of Inflammasome Activation in Response to Yersinia Infection by Fluorescence Microscopy Detection of Active Caspase-1 Puncta (Erin E. Zwack, Igor E. Brodsky)....Pages 231-240
Methods for Detection of Pyrin Inflammasome Assembly in Macrophages Infected with Yersinia spp. (Natasha P. Medici, James B. Bliska)....Pages 241-255
Back Matter ....Pages 257-259

✦ Subjects

Life Sciences; Bacteriology; Pathology; Infectious Diseases