Pathogenesis of low dose streptozotocin induced diabetes in mice: requirement for α1-adrenoceptor activation and vasoactive amine release
✍ Scribed by S. Martin; V. Kolb-Bachofen; U. Kiesel; H. Kolb
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 302 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0012-186X
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✦ Synopsis
Pancreatic islet inflammation and subsequent diabetes was induced by multiple low doses of streptozotocin in male C57 B1/6J mice. The development of hyperglycaemia was almost completely prevented by treating the animals with the cd-adrenoceptor antagonist prazosin (20mg.kg -1. day -1) as well as by the vasoactive amine antagonists methysergide (50 mg. kg-1. day-1), disodium cromoglycate (100 mg. kg-1. day-1), pizotifen (5 mg. kg-1. day-1) or cyproheptadine (20 mg. kg-1. day-1). Treatment with vasoactive amine antagonists largely inhibited infiltration of pancre-atic islets by L3T4+-lymphocytes and to a lesser extent by Lyt2 +-cells. The infiltration of macrophages was not affected except after pizotifen treatment. These results indicate that al-adrenoceptor activation is required for disease development and that vasoactive amine release is a prerequisite for lymphocytic insulitis but not for macrophage infiltration of islets.