Patatin, a major soluble protein of the potato (Solanum tuberosumL.) tuber is synthesized as a larger precursor

✍ Scribed by B. Krischner; H. Hahn

• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 671 KB
• Volume: 168
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00392365

✦ Synopsis

Antibodies were raised against the highly purified glycoprotein patatin. They were used to characterize the product synthesized in a wheatgerm cell-free translation system, programmed with polyadenylated RNA from potato tubers. Sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed that the immunoprecipitated protein had a molecular mass of 43 kDa compared to 40 for the authentic patatin. It is assumed that patatin is synthesized in vivo as a larger precursor which is processed to the mature protein by cleavage of a signal peptide. Our results are in agreement with sequence-analysis data of patatin complementary DNA which indicate a signal peptide of about 23 amino acids (Mignery et al., 1984; Nucleic Acids Res. 12, 7987-8000).