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Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions

✍ Scribed by Beers, Jeanette; Gulbranson, Daniel R; George, Nicole; Siniscalchi, Lauren I; Jones, Jeffrey; Thomson, James A; Chen, Guokai

Book ID
115547398
Publisher
Nature Publishing Group
Year
2012
Tongue
English
Weight
753 KB
Volume
7
Category
Article
ISSN
1750-2799

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## Abstract The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA