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Partitioning behaviour in aqueous two-phase systems and fractionation by counter-current distribution of chick-embryo erythrocytes with numerical resolution of distributiion curves

✍ Scribed by Estrella Ferrer; Margarita Martin; Ramón Alonso; José Luque

Book ID: 104145747
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 682 KB
Volume: 445
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)84545-3

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✦ Synopsis

The partition of chick-embryo and young-chick erythrocytes in dextran-poly-(ethylene glycol) two-phase systems depends on the interfacial tension and electrical potential differences between the phases. Counter-current distribution with charged 5% dextran-poly(ethylene glycol) systems has proved to be an adequate method for the separation of primitive and definitive erythrocytes present in chick embryos when a phase settling time of 20 min is used. The computer-aided numerical resolution of experimental curves has shown the existence of subpopulations which could not have been detected by using conventional methods.

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