This study examines the particle size characteristics of hillslope soils and fluvial suspended sediments in an agricultural catchment. Samples of surface runoff and stream flow were collected periodically and analysed for the size distributions of the effective (undispersed) sediment. This sediment was subsequently dispersed and the ultimate size distributions determined. The median effective particle size of stream suspended sediment was considerably coarser than the median ultimate particle size, indicating that most of the load included a substantial proportion of aggregates. Moreover, the proportion of fine material (i.e. silt and clay) increased, and the proportion of sand-sized material decreased, with increasing discharge. This decrease in sediment size with increased flow, which is contrary to the traditional assumption of a positive discharge/particle size relationship, is thought to reflect: (i) the influx of silt and clay, predominantly the former, originating on the catchment slopes and brought to the stream by overland flow along vehicle wheelings, roads and tracks; and (ii) erosion of fine material from the channel bed and banks. During large storms, however, the proportion of sand-sized sediment increased during the rising limb of the hydrograph, as a result of the entrainment of coarser source material from the valley floor during overbank flooding. The stream suspended sediment was finer than the catchment soils and considerably finer than material eroding from the catchment slopes during storms. The degree of clay and silt enrichment in the suspended sediments was largely the result of preferential deposition of the coarser fraction during the transport and delivery of sediment from its source to basin outlet. The data from this study confirm that a significant mode of sediment transport in fluvial systems is in the form of aggregates, and that the dispersed sediment size distribution is inappropriate for determining the transportability of sediment by flow.