Particle motion in single acinar cells observed by microscope laser light scattering spectroscopy
✍ Scribed by J. A. Peetermans; E. K. Matthews; I. Nishio; T. Tanaka
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 441 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1432-1017
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✦ Synopsis
We report here the direct observation of the motion of cytoplasmic subcellular organelles and macromolecules within single, metabolically active, pancreatic acinar cells using microscope laser-light scattering spectroscopy (MLLSS). The relative amount and the "effective diffusion coefficient" of the mobile particles shows a marked difference in magnitude between the apical and basal regions of the cell. Secretory stimulation evoked by the cholinergic agonist bethanechol induces changes in the relative motion in the cytoplasm of approximately one fifth of the acinar cells. This study demonstrates the feasibility and utility of a novel technique, MLLSS, for the analysis of intracellular events in regions as small as (2 micron)3 in single, granule secreting cells. It also shows MLLSS to be a powerful tool for the detection and measurement of altered motion in discrete subcellular regions of small mammalian cells after biochemical and pharmacological manipulations.