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PARTICIPATION OF THE Ca2+-CALMODULIN-ACTIVATED KINASE II IN THE CONTROL OF METAPHASE-ANAPHASE TRANSITION IN HUMAN CELLS

✍ Scribed by Christian P. Petzelt; Sodikdjon Kodirov; Grit Taschenberger; Wolfgang J. Kox

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
1011 KB
Volume
25
Category
Article
ISSN
1065-6995

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✦ Synopsis

Treatment of HeLa S3 cells growing in suspension, and of endothelial cells ECV 304 growing as a monolayer, with the inhibitor of the Ca^2+^‐calmodulin activated Kinase II KN‐93, blocks cells at metaphase for 15min (HeLa cells) and 30min (ECV cells). Thereafter cells resume mitosis and enter anaphase. The inactive isomer KN‐92 does not show such effects. The results show the involvement of the CaM K II system in the regulation of the metaphase‐anaphase transition whereby the activation of the Kinase II, in particular by calmodulin appears to be affected, the residual autophosphorylation of the CaM K II system apparently sufficing after 15 to 30min to release the cells into anaphase. The results are compared with the metaphase‐blocking effects of the noble gas xenon, where the xenon‐induced block can be overcome by small intracellular increases of Ca^2+^, thus indicating the CaM K II system as a possible target for xenon.

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