Participation of a sialic acid-specific lectin from freshwater prawnMacrobrachium rosenbergii hemocytes in the recognition of non-self cells
✍ Scribed by Vázquez, Lorena; Maldonado, Guadalupe; Agundis, Concepcion; Pérez, Armando; Cooper, Edwin L.; Zenteno, Edgar
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 228 KB
- Volume
- 279
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Phagocytic activity of circulating hemocytes from freshwater prawns is mediated by a small group of granulocytes. Recognition of nonself cells by these cells seems to be mediated by two independent mechanisms: specific, via O-acetylsialic acid, as well as N-acetylated sugars on recognized cells and a nonspecific one. Both mechanisms show differences in their optimal temperature and time differences for activity. Hemocytes with phagocytic activity interact positively with rabbit IgG raised against the serum lectin. Attempts to elucidate the nature of the membrane-lectin on hemocytes performed by electroimmunotransfer blot assays on cell lysates indicates the presence of two major proteins with a molecular weight of 68-72 kDa. Electron microscopy revealed a regular distribution of lectin on hemocyte plasma membranes. Our results suggest the active participation of a membrane lectin in the recognition of nonself. J.