Partial resolution of mandelic acid with Sephadex gels

✍ Scribed by Robert E. Leitch; Herbert L. Rothbart; Wm. Rieman III

• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 331 KB
• Volume: 28
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85940-9

✦ Synopsis

NOTES 3MM

). Elution was with phenol-water (3 : z, w/w). After colouring with the periodate-Schiff reagent we found a single spot in each fraction. In the GDG-fraction, a spot with RF 0.68 (= monogalactosyglycerol) was found and in the GGDG-fraction the spot had RF 0.51 (digalactosylglycerol) . This demonstrated that : (I) The phospholipid fraction contained no glycolipids.

(2) The GDG-fraction contained no phospholipids or GGDG.

(3) The GGDG-fraction was free of GDG and phospholipids. Most of the plant p:gments were removed by this method. Wowever, the glycolipid fractions contained some yellow-brown pigments.