Partial purification of the activated hepatic glucocorticoid-receptor complex
โ Scribed by Colman, Paul D.; Feigelson, Philip
- Book ID
- 122871193
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 481 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0303-7207
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๐ SIMILAR VOLUMES
## Abstract A crucial step in the interaction of glucocorticoids with target cells is the activation step, which involves a conformational change in the cytoplasmic glucocorticoidโreceptor protein complexes and facilitates their binding to the cell nucleus. Activation can be quantified by measuring
Protamine sulfate was found to precipitate completely the nonactivated [3H]dexamethasone-receptor complex of rat liver. This observation was then used as the basis of a method to separate activated from nonactivated complex. Thus, addition of 10 mg/ml of protamine sulfate to the rat hepatic cytosol