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Partial purification of mitochondrial and supernatant DNA polymer from saccharomyces cerevisiae

✍ Scribed by Akio Iwashima; Murray Rabinowitz

Book ID
115732107
Publisher
Elsevier Science
Year
1969
Weight
643 KB
Volume
178
Category
Article
ISSN
0005-2744

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Purification of DNA-dependent RNA polyme
Purification of DNA-dependent RNA polymerase II from Saccharomyces cerevisiae
✍ Grant A. Bitter πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 807 KB

A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a