✦ LIBER ✦
Partial purification and properties of extracellular chitinase produced byAcremonium obclavatum, an antagonist to the groundnut rust,Puccinia arachidis
✍ Scribed by K. R. Gunaratna; R. Balasubramanian
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 295 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1573-0972
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✦ Synopsis
An extracellular chitinase of Acremonium obclavatum was partially purified. It had an M r of 45 kDa on SDS-PAGE, and was optimally active at pH 3 to 4 and 50°C. Hg and Mn (10 mM) inhibited activity. The chitinase hydrolysed colloidal chitin more rapidly than crude chitin or isolated A. obclavatum cell walls. The partially-purified enzyme inhibited uredospore germination and germ-tube growth of Puccinia arachidis.