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Partial purification and kinetic characterization of transaldolase fromDictyostelium discoideum

โœ Scribed by Kathy R. Albe


Book ID
119078608
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
760 KB
Volume
15
Category
Article
ISSN
0147-5975

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Purification and properties of the NADP-
โœ F. Pamula; J. F. Wheldrake ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 523 KB

NADP-dependent glutamate dehydrogenase from Dictyostelium discoideum was purified 9300 fold with a yield of 4.6%. The enzyme is a hexamer of apparent molecular weight 294 kDa on Sephacryl S400 and a subunit molecular weight of 52 kDa as determined by SDS gel electrophoresis. The apparent Kms for alp