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Partial purification and characterization of immunoglobulin production stimulating factor derived from namalwa cells

โœ Scribed by Koji Yamada; Kazuhiko Akiyoshi; Hiroki Murakami; Takuya Sugahara; Ichiro Ikeda; Kazuhisa Toyoda; Hiroshisa Omura

Book ID
112801056
Publisher
Society for In Vitro Biology
Year
1989
Tongue
English
Weight
481 KB
Volume
25
Category
Article
ISSN
1475-2689

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โœ Kazuhisa Toyoda; Takuya Sugahara; Kunio Inouye; Koji Yamada; Sanetaka Shirahata; ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 717 KB

## An immunoglobulin (Ig) production stimulating factor (IPSF) for hybridomas was found in spent medium of the human B lymphoblastoid cell line, HO-323. The IPSF was purified by serial use of DEAE chromatography, ultrafilnation, gel filtration and HPLC-DEAE chromatography. Purified IPSF was estim