Partial Protection against Botulinum B Neurotoxin-Induced Blocking of Exocytosis by a Potent Inhibitor of Its Metallopeptidase Activity
✍ Scribed by Christine Anne; Serge Turcaud; Armand G. S. Blommaert; François Darchen; Eric A. Johnson; Bernard P. Roques
- Book ID
- 101816119
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 107 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1439-4227
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Clostridium botulinum neurotoxins (BoNTs) cause botulism, which is characterized by a flaccid paralysis, through inhibition of acetylcholine release by peripheral cholinergic nerve terminals. This is due to the zinc metallopeptidase activity of the neurotoxin, cleaving one component (synaptobrevin for BoNT/B) of the exocytosis machinery. Yet, there are no specific agents able to control the peptidase‐related effects of BoNT/B. We recently developed the first compounds to inhibit this enzymatic activity in the nanomolar range. Here we report that two of our best inhibitors prevent the BoNT/B‐induced cleavage of native synaptobrevin on synaptic vesicles, and partially inhibit the suppression of [^3^H]noradrenaline release from synaptosomes that is caused by BoNT/B. These results were obtained at micromolar concentrations, consistent with the measured inhibitory potency of these inhibitors on the native toxin. These compounds provide a new way to possibly prevent and/or to control the neurotoxin effects of botulinum.