✦ LIBER ✦
Partial isotope fractionation during high-performance liquid chromatography of deuterium-labelled internal standards in plant hormone analysis: A cautionary note
✍ Scribed by B. H. Brown; S. J. Neill; R. Horgan
- Book ID
- 104752515
- Publisher
- Springer-Verlag
- Year
- 1986
- Tongue
- English
- Weight
- 234 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Deuterium-labelled indole-3-acetic acid, abscisic acid and phthalimido-l-aminocyclopropane-l-carboxylic acid were found to separate from the unlabelled compounds on reverse-phase high-performance liquid chromatography (HPLC). A similar separation was found for the methyl esters of these compounds on normal-phase HPLC. Such separations may lead to substantial errors when these compounds are used as internal standards for quantitation by gas chromatography-mass spectrometry/selective ion detection, unless the complete chromatographic peaks are collected.