Partial characterization of the mating-inducing factors (gamones) produced by mating type VI cells in the ciliateEuplotes patella syngen 2
✍ Scribed by Akada, Rinji
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 337 KB
- Volume
- 237
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
The mating-inducing factors (gamones) secreted from the mating type VI (strain 10) cells in the ciliate Euplotes patella syngen 2 have been characterized. The gamone activity was sensitive to pronase and was resistant to trypsin, chymotrypsin, heating, and periodate oxidation. DEAE-Sepharose column chromatography of the cell-free fluid has revealed that cells of heterozygous mating type VI (mt2/mt4) secrete two different gamones, gamones 2 and 4. These results strongly suggest that the two gamones of type VI cells are proteins that contain little or no carbohydrate moiety and are controlled by their own mating type genes.