Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor

✍ Scribed by Janeczek, Jutta ;Born, Jochen ;Hoppe, Peter ;Tiedemann, Hildegard

• Book ID: 104656384
• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 780 KB
• Volume: 201
• Category: Article
• ISSN: 1432-041X
• DOI: 10.1007/bf00188773

✦ Synopsis

High (Mrs90 110 kDa) and low (Mr~15-30 kDa) molecular weight forms of neural-inducing factors have been found in the supernatant of Xenopus gastrula homogenate. The factors, which are protein in nature, have been partially purified by size exclusion highperformance liquid chromatography (HPLC) and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The factor of smaller size, which could be derived from a precursor, is associated with other proteins in a larger complex. The neural-inducing factors are not irreversibly inactivated after chemical deglycosylation with trifluoromethansulfonic acid. The neural-inducing protein which is found in ribonucleoprotein (RNP)-particles was partially purified by hydrophobic chromatography. Possible relationships of the factors in different subcellular fractions and their physiological significance are discussed.