Part E: Hormone Action

✍ Scribed by Bert W. O'Malley, Joel G. Hardman (Eds.)

• Publisher: Academic Press
• Year: 1975
• Tongue: English
• Leaves: 419
• Series: Methods in Enzymology 40
• Edition: 1
• Category: Library

✦ Synopsis

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences

✦ Table of Contents

Content:
Contributors to volume XL
Pages vii-viii

Preface
Page ix
Bert W O'Malley, Joel G Hardman

Volumes of series
Pages xi-xiv

[1] Techniques of localization of proteins and nucleoproteins in the cell nucleus by high resolution autoradiography and cytochemistry Original Research Article
Pages 3-41
M Bouteille, A.M Dupuy-Coin, G Moyne

[2] A nuclear system for DNA replication from HeLa cells Original Research Article
Pages 41-44
Daniel L Friedman

[3] Analysis of the actionof chemical agents upon the cell division cycle Original Research Article
Pages 44-57
James B Kurz, Daniel I Friedman

[4] Methods for analysis of cell cycles in vivo Original Research Article
Pages 58-62
S.H Socher

[5] Electron microscopy and autoradiography of chromosomes Original Research Article
Pages 63-75
Elton Stubblefield

[6] Parallel isolation procedures for metaphase chromosomes, mitotic apparatus, and nuclei Original Research Article
Pages 75-89
Wayne Wray

[7] Fractionation of Chromatin Original Research Article
Pages 93-97
Delill S Nasser, Brian J McCarthy

[8] Isolation of template active and inactive regions of chromatin Original Research Article
Pages 97-102
James Bonner, Joel Gottesfeld, William Garrard, Ronald Billing, Lynda Uphouse

[9] Methods for analysis of histones Original Research Article
Pages 102-138
Lubomir S Hnilica

[10] Methods for the assessment of selective histone phosphorylation Original Research Article
Pages 138-144
Rod Balhorn, Roger Chalkley

[11] Methods for isolation and characterization of nonhistone chromosomal proteins Original Research Article
Pages 144-160
Sarah C.R Elgin

[12] Methods for isolation and characterization of chromosomal nonhistone proteins: Fractionation of chromatin on hydroxyapatite and characterization of the nonhistone proteins by ion exchange chromatography and polyacrylamide gel electrophoresis Original Research Article
Pages 160-171
A.J MacGillivray, D Rickwood, A Cameron, D Carroll, C.J Ingles, R.J Krauze, J Paul

[13] Methods for isolation and characterization of acidic chromatin proteins Original Research Article
Pages 171-176
Elizabeth M Wilson, Thomas C Spelsberg

[14] Methods for analysis of phosphorylated acidic chromatin protein interactions with DNA Original Research Article
Pages 177-191
Lewis J Kleinsmith, Valerie M Kish

[15] Immunochemical characteristics of chromosomal proteins Original Research Article
Pages 191-198
F Chytil

[16] Chromatin protein kinases Original Research Article
Pages 198-208
Valerie M Kish, Lewis J Kleinsmith

[17] Circular dichroism analysis of nucleoprotein complexes Original Research Article
Pages 209-238
Thomas E Wagner, Vaughn Vandegrift, Dexter S Moore

[18] Methods for analysis of enzyme synthesis and degradation in animal tissues Original Research Article
Pages 241-266
Robert T Schimke

[19] Enrichment of polysomes synthesizing a specific protein by use of affinity chromatography Original Research Article
Pages 266-273
E Brad Thompson, J.V Miller Jr.

[20] The use of inhibitors in the study of hormone mechanisms in cell culture Original Research Article
Pages 273-293
Walter A Scott, Gordon M Tomkins

[21] The design of double label radioisotope experiments Original Research Article
Pages 293-302
Edwin D Bransome Jr.

[22] Use of antibodies to nucleosides and nucleotides in studies of nucleic acids in cells Original Research Article
Pages 302-307
B.F Erlanger, W.J Klein Jr., V.G Dev, R.R Schreck, O.J Miller

[23] Techniques for the study of hormone effect on collagens Original Research Article
Pages 307-377
Dorothy H Henneman, George Nichols Jr.

Author index
Pages 379-393

Subject index
Pages 394-404