Part 2.N-[m-(3-(trifluoromethyl)diazirine-3-yl)phenyl]-4- (-3-thio(-1-D-galactopyrannosyl)-maleimidyl)butyramide (MAD-Gal) on diamond

✍ Scribed by L�onard, D.; Chevolot, Y.; Bucher, O.; Haenni, W.; Sigrist, H.; Mathieu, H. J.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 354 KB
• Volume: 26
• Category: Article
• ISSN: 0142-2421
• DOI: 10.1002/(sici)1096-9918(199810)26:11<793::aid-sia421>3.0.co;2-k

✦ Synopsis

Time-of-Ñight secondary ion mass spectrometry (ToF-SIMS) and x-ray photoelectron spectroscopy (XPS) are used to characterize a newly synthesized glycosylated photoactivatable reagent designed for surface glycoengineering. The glycoaryldiazirine reagent diazirine-3-yl)phenyl ] -4-(-3-thio(-1-Dgalactopyrannosyl)-maleimidyl)butyramide) is immobilized with 350 nm light on diamond surfaces. The XPS atomic constituents and chemical shifts, as well as ToF-SIMS-speciÐc characteristic fragments of the photoimmobilized molecule, are identiÐed. The grafting on oxygen-containing sites previously observed for N-(m-(3-(triÑuoromethyl)diazirine-3-yl)phenyl)-4-maleimido-butyramide (MAD) is conÐrmed. The di †erence in grafting efficiency for fresh and aged substrates indicates that other still unidentiÐed reaction sites could be involved. The ToF-SIMS imaging is used to exhibit the ability to pattern the surface with the glycosylated aryldiazirine.