[Part 2: Biological Sciences] || DNA Sequence of the Serratia marcescens Lipoprotein Gene
โ Scribed by Kenzo Nakamura and Masayori Inouye
- Book ID
- 123665934
- Publisher
- National Academy of Sciences
- Year
- 1980
- Tongue
- English
- Weight
- 937 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/8458
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๐ SIMILAR VOLUMES
The cloned ompA gene from Serratia marcescens was fully expressed in Escherichia coli and its product correctly assembled into the outer membrane. The S. marcescens polypeptide was not functionally equivalent to the E. coli OmpA protein, which serves as a phage receptor and as a component of several
A pBR322-derived plasmid pKEN221 carrying a Serratia marcescens lpp gene overproduces the outer membrane lipoprotein in an Escherichia coli lpp- cell. However, when this strain was continuously cultured in a rich medium for about thirty generations, many Lpp- mutants were accumulated. Out of six mut