Mating in Yarrowia lipolytica can be induced by mixing cells of both complementary mating types (A and B) in yeast extract-malt-medium. By means of an inbreeding programme, strains with significantly improved mating frequencies were selected. The main events of the mating process, that have been det
Parasexual process in the yeast Yarrowia lipolytica
β Scribed by Dr. Cornelia Kurischko
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 512 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0233-111X
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β¦ Synopsis
Genetic studies of several events of the life cycle of Y. Eipolyte'ca demonstrated that diploid strains were unstable and produced mitotic segregants by haploidization. A screening system was developed which enabled us to show that parasexual processes can take place in addition to the sexual life cycle. This haploidization occurred through aneuploid intermediates as was proven statistically by the deviations from the segregation pattern as well as by the segregation data of the clones.
The direction of the cross, waswith respect to the resistance to 2-deoxyglucose of A-or B-strain not important for selection of mitotic segregants.
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π SIMILAR VOLUMES
By means of special selective conditions we analyzed the temporal relationship of diploidization and haploidization in the yeast Yarrowia lipolytica. These processes could be divided into several steps. Twelve hours after mixing parental strains dissociation products of unstable heterokaryons are se