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Parallel screening of selective and high-affinity displacers for proteins in ion-exchange systems

✍ Scribed by Kaushal Rege; Asif Ladiwala; Nihal Tugcu; Curt M. Breneman; Steven M. Cramer


Book ID
108092024
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
223 KB
Volume
1033
Category
Article
ISSN
1873-3778

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## Abstract A library of cold shock protein B (CspB) mutant variants was employed to study protein binding affinity and preferred orientations in cation exchange chromatography. Single site mutations introduced at charged amino acids on the protein surface resulted in a homologous protein set with